Evaluation of the efficacy of leave Extract of Ziziphus spina-Christi against three Bacterial species

الملخص

Background: Plants hold immense significance in our existence as they cater to our fundamental requirements for nourishment, shelter, attire, energy, decorative elements, seasoning, and healing. The family Rhamnaceae encompasses the intriguing genus Ziziphus. This remarkable genus consists of approximately 100 species of either deciduous or evergreen trees and shrubs, thriving in the tropical and subtropical regions across the globe.

Aims: the present study aimed to evaluate the leaves extracts of Z. spina-christi against three Bacterial species.

Results: The mean values for the diameters and zones of growth inhibition created by the extracts of Z. spina-christi leaves against pseudomonas sp were noted as (161.27, 33.73, and 44.81 mm²) for the region of activity when concentrations of leaves extract (100, 50, and 25 ml) were utilized in sequence. Conversely, the activity area observed was (286.70, 25.20, and 395.44 mm²) when the antibiotics Streptomycin, Ampicillin, and Gentamycin were introduced. Additionally, the aqueous extracts of Z. spina-christi yielded measurements of (133.68, 104.72, and 17.141 mm²) for the active area when the leaf extract concentrations (100, 50, and 25 ml) were administered respectively. Meanwhile, the recorded area of activity was (306.81, 168.70, and 134.91 mm²) when Streptomycin, Ampicillin, and Gentamycin were employed against staphylococcus sp. Furthermore, the aqueous extracts of Z. spina-christi displayed values of (96.89, 42.17, and 20.49 mm²) for the area of activity when the leaf extract concentrations (100, 50, and 25 ml) were applied accordingly. On a different note, the recorded area of activity reached (320.94, 21.38, and 42.17 mm²) when the antibiotics Streptomycin, Ampicillin, and Gentamycin were used against klebsella sp.

Conclusion: Gentamycin showcases unparalleled potency against Pseudomonas sp among the antibiotics examined, while the 100ml extraction of Z. spina-christi leaves exhibits the most remarkable effect among its various concentrations. Streptomycin stands out as the most effective against Staphylococcus sp within the realm of studied antibiotics, with the 100ml Z. spina-christi leaves extraction demonstrating the highest efficacy compared to other concentrations. Streptomycin also reigns supreme against Klebsella sp among the reviewed antibiotics, as the 100ml extraction of Z. spina-christi leaves reveals the strongest impact among the available concentrations.